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The course of action that is routinely used for the differential fractionation of liver tissue serves like a easy illustration of this technique and is also demonstrated diagrammatically in Figure 12-4.
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In certain cases, it is not important to layer the sample to become fractionated onto a preformed density gradient. As a substitute, the sample and the density gradient answers are combined and positioned during the centrifuge tube. Throughout centrifugation at large pace, the density gradient varieties quickly within the tube, and particles migrate (upward and/or downward) to their isopycnic positions.
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